Lab Report 4 DNA Isolation

 

dna lab report

A classroom pre-laboratory activity that introduces the concept of the cell and DNA. A DNA isolation laboratory activity that allows students to extract and visualize DNA from different types of cells (plant and animal) with an introduction to scientific procedures. DNA lab report - Orett White Jordan Trecki Analysis and In order to extract the DNA we obtained a bacterial suspension of E-Coli in saline EDTA, and treated it with various amounts of chemicals and then centrifuged this mixture in order to create a mixture in which DNA was located in the top aqueous layer%(16). Lab Report: DNA Isolation. Alcohol is used to precipitate the DNA which is not soluble in alcohol. The salt helps the DNA strands come together. and it releases the DNA. The solution is made of water. Principles: 1. 4. Aim: To observe DNA by isolating it from a cell. and salt to free the DNA 5/5(3).


Agarose Electrophoresis Lab Report | Gel Electrophoresis of DNA :: Custom Papers Writing Help


This preview shows page 1 - 5 out of 9 pages. Subscribe to view the full document. I cannot even describe how much Course Hero helped me this summer. In the end, I was not only able to survive summer classes, but I was dna lab report to thrive thanks to Course Hero. Temple University. The question to be answered was exactly how pure was the extracted chromosomal DNA? Given this fact it dna lab report possible that some contamination took dna lab report during the extraction phase of the experiment.

This contamination could have resulted in some impurities in the final DNA sample. Method: The method was in variety of steps. The sample was first denatured, dna lab report, treated with a variety of substance for comparison.

The samples were then stained using ethidium bromine and then placed in agarose gel. An electrical current was then run through the gel to separate and analyze the nucleic acids Materials and Methods: See attached protocol for more information. Lanes seven and eight were switched. It is present in all organisms on the planet.

DNA is important because it give the organism the layout for all proteins in the body. During the lab the purpose of the experiment was to extract chromosomal DNA from E-coli bacteria and then analyze the extracted DNA by using agarose gel electrophoresis. This hypothesis was brought about because with the equipment available to us and the large concentrations of RNA present with the DNA, it would be almost impossible to not have some impurities in our extracted sample, dna lab report.

In order dna lab report extract the DNA we obtained a bacterial suspension of E-Coli in saline EDTA, and treated it with various amounts of chemicals and then centrifuged this mixture in order to create a mixture in which DNA was located in the top aqueous layer, dna lab report.

We then treated the DNA in various percentages of ethanol in order to further purify it. We then loosened the DNA by placing it in STE and then placed it in a microfuge tube and stored it in a freezer for a week in order to prepare it for analysis. In the next part of dna lab report experiment we used our extracted DNA and used it to created 6 preps with various amounts of DNase, water and buffer all at various temperatures, as well as creating two preps of tRNA.

Each prep was immersed in a solution of ethidium bromide a mutagen which diffuses into the gel and tightly binds to nucleic acid. We then. Share this link with a friend: Copied!

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Agarose Gel Electrophoresis of DNA Lab Report Example | Topics and Well Written Essays - words

 

dna lab report

 

A classroom pre-laboratory activity that introduces the concept of the cell and DNA. A DNA isolation laboratory activity that allows students to extract and visualize DNA from different types of cells (plant and animal) with an introduction to scientific procedures. Dec 08,  · LAB REPORT 6 (BACTERIAL DNA EXTRACTION) INTRODUCTION. DNA isolation is a process of purification of DNA from sample using a combination of physical and chemical methods. DNA is extracted from human cells for a variety of reasons. With a pure sample of DNA we can test a newborn for a genetic disease, analyze forensic evidence, or study a gene Author: IBGREPORTGROUP Extracting DNA from a Banana and Other Fruits. Introduction. All living things, bananas and people included, pass on information from one generation to the next using the same basic material, DNA. Within every living organism, most cells contain a complete set of DNA instructions. The information in DNA tells our bodies how to develop, grow, and work.